Procedure for the Operation and Calibration of Humidity Chamber

This document details the procedure for the Operation and Calibration of Humidity Chamber

It is the policy of Xyz Limited that a written procedure shall be followed the Operation and Calibration of Humidity Chamber and its use monitored in order to ensure smooth operation and its use monitored to obtain consistent and reproducible results and to minimize downtime and to meet regulatory requirements.

This procedure is to be applied at the time of Operation and Calibration of Humidity Chamber.


Persons along with their responsibilities and accountability are given below:

Executive – Corporate Quality Assurance, To prepare the SOP. To maintain the records as per SOP and Trainee Analyst/ Jr. Analyst/ Analyst/ Sr. Analyst, To follow the SOP accordingly and To ensure implementation of SOP Manager – Quality Assurance.



5.1.1 Check and ensure that:

5.1.2 The Equipment is clean and free from dust.

5.1.3 There should not be any powder/material inside and the chamber should be free from dust.

Check the availability of water and its label in the boiler tank.

The boiler heater pin should be connected to the boiler heater.

There should not be any clogging between the storage tank and the boiler tank.


5.2.1 Switch `ON’ the mains switch on the control panel 

5.2.2 Set the specified temperature (30°C) on the temperature controller of the chamber with the help of the Temperature controller by pressing up or down keys.    

The bottom SV (Set Value) display shows the set temperature and the top PV (Process Value) display will show the actual temperature.

Set the humidity on the humidity controller of the chamber 65 % RH with the help of the Humidity controller by pressing up or down keys.

5.2.5 The bottom SV (Set Value) display shows the set Humidity. And the top PV (Process Value) display will show the actual Humidity

5.2.6 Set the digital safety controller to 30°C. The safety controller cut off the main contactor in case of overshoot or undershoot of the temperature by 5°C. In case of this type of trip restart the chamber by pressing the pushbutton switch on the control panel.

5.2.7 Allow the chamber to attend the specified temperature


5.3.1 PC / Printer interface unit is applicable for the recording of the data of the chamber at a specified time period of time and to connect the chamber to PC through the computer software.

5.3.2 To view the specified parameters, press and “SEL” key and view the parameters.

5.3.3 To change the parameters press and hold the “SEL” key till “PASSWORD”  displays.

5.3.4 Select the password by pressing the up key.  Or Down key  

5.3.5. Now select the desired parameter using the “SEL” key and press enter key.

5.3.6 Chang the desired parameter by pressing the up or down key as per the requirement and pressing enter key to confirm the same.

5.3.7 Again set other parameters like date & time, time interval, printer setting etc. using the same  

5.3.8 Finally select the parameter “LOG OUT” using the “SEL” key and press the enter key to save the selected parameter.

5.3.9 To view the data select the display channel.

Procedure for the Operation and Calibration of Humidity Chamber


5.4.1  Run the Thermolab data acquisition software “enviro” by double-clicking on the icon.

5.4.2 Software will open with a detailed display, which shows the reading received from the chambers.

If NA (Not Applicable) is displayed for any of the Chamber it means the chamber is not connected.

Select the Desired Chamber No. of which the current reading is required to display in current reading windows.

Minimum and Maximum limits can be set by the red colour icon.

To download the data select the green coloured icon to download on position, the data acquired will lead to the system.

5.4.7 To view or to take the printout of the data of a specific period of time select the date and time and then press Graph or Report.

5.4.8 The graph and or report of the temperature and/or humidity will display according.

5.4.9 Press print to get the printout or select  key to exit in case of the graph and select “close” key to close the report format

5.5 Maintain the logbook as per Appendix I of  SOP   XYZ/CQA/SOP-016 (Stability of Finished Products)   


6.0.1 Calibration Frequency: Half-yearly

6.0.2 Calibrated Digital hygrometer is used for calibration of the Humidity chamber.

6.0.3 Insert the hygrometer as near as possible to the sensor of the Humidity chamber

6.0.4 Set the temperature first at 30°C & RH 65 %.

6.0.5 When the temperature is stable note down the temperature reading as displayed by Humidity Chamber and reference hygrometer (used for calibration) simultaneously.

6.0.6 Record the temperature and humidity readings at 1 hour, 2hour, and 3 hours.

6.0.7 Record the calibration data  on XYZ/CQA/SOP-071/FR-01 as Calibration Datasheet Appendix I

6.0.8 After calibration of the instrument, affix the calibration sticker on the instrument and fill in all the required details as per SOP No.XYZ/CQA/SOP-006, Labelling policy.

6.0.9 Report any discrepancy observed during operation and calibration of instrument to Section Incharge or his representative for corrective and preventive action as per SOP No. XYZ/CQA/SOP-030.


6.1.1 Carry out the validation of the chamber to check the temperature distribution at different locations, once in a year or after any major repairs as per respective validation protocol by an external agency or In the house.

6.1.2 In case of validation fails to meet the desired specifications/criteria, call the service engineer/maintenance personnel. Also, put a label on equipment indicating its breakdown.  


Generate the instrument calibration number on the calibration datasheet as INSCALXXYYZZZ where INS denotes instrument, CAL denotes calibration, XX denotes the year, YY denotes month and ZZZ denotes sequence number.


XYZ                  XyzLimited

SOP                 Standard Operating Procedure

QC                   Quality Control

CQA                Corporate Quality Assurance 

>Procedure for the Operation and Calibration of LC/MS/MS


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