1.1 To lay down the procedure is to provide guidelines for the LAMINAR AIR FLOW operation.
2.0 SCOPE
2.1 This procedure is applicable for LAF which is installed in the Microbiology laboratory of the Quality Control Department of XYZ Ltd.
3.0 RESPONSIBILITIES
3.1 Microbiologist to prepare and follow
the SOP.
3.2 Head – QA & QC for implementation
of SOP.
4.0 Procedure of laminar air flow
4.1 Operation
and Cleaning of LAF.
4.2 Switch
“OFF” the UV light.
4.3 Clean the equipment
with 70% IPA solution.
4.4 Switch
“ON” the “AIR” and Visible light switch situated on the control panel on the right-hand
side of the instrument.
4.5 Start the
spirit lamp and carry out the work.
4.6 After
completion of work switch OFF the Airflow and Spirit lamp.
4.7 Clean any
remaining water or waste liquid material spilled on the laminar airflow
platform properly with dry cotton.
4.8 Clean
properly the working chamber from the ceiling, followed by side glass and then
the platform of the chamber with 70% IPA.
4.9 Spray
sterile 70% IPA and switch OFF the visible light and the Switch ON the UV light
till to start the next operation.
5.0 Precautions of Laminar Air flow operation
5.1 Take care
to prevent any damage to the integrity of the filter during cleaning. In case
of observation of any damage to filters, immediately inform your senior for
further action.
5.2 Instrument
should be cleaned when the electrical connections of the equipment are in OFF
position.
5.3 Clean the
LAF chamber after every operation.
5.0 ABBREVIATIONS
LAF: Laminar Air Flow
SOP: Standard Operating Procedure
QA: Quality Assurance
QC: Quality Control
6.0 REFERENCE:
6.1 Instruction
manual
