The importance of SOP for water analysis in the industry and testing procedure or methods DM water. Refined Water is the main crude material utilized in drug-producing. Because of the basic idea of water and water frameworks, Purified Water Testing is an administrative necessity to perform routine microbiological and substance quality appraisals.
1.0 Â OBJECTIVE
1.1 To Lay down the Procedure for the Analysis of DM Water.
2.0 Â SCOPE
2.1 This procedure is applicable to the Analysis of  DM Water  of quality Controlled in QC Lab
 3.0  RESPONSIBILITIES
3.1Â Â Â Â QC Chemists Lab analyst follows the SOP.
3.2Â Â Â Â Head- QA & QC for implementation of SOP.
4.0Â DM WATERÂ SAMPLING Â PROCEDURE
Collect the Sample as per Standard Operating. Procedure for water sampling and analysis
For Chemical Parameter as per their specification.
Purified water testing as per USP | Purified water testing as per IP
5.0 Â Water quality testing procedures.
Chemical analysis methods used for water analysis
Purified water chemical analysis |Â Standard analytical procedure for water Analysis.
Acidity or alkalinity: To 10 ml, freshly boiled & cooled in a borosilicate glass flask, add 0.05 ml of methyl red solution, the resulting solution is not red. To 10 ml add 0.1 ml of bromothymol blue; the resulting solution is not blue.
Ammonium in water: To 20 ml add 1 ml of alkaline potassium mercury iodide solution & allow to stand five minutes. When viewed vertically the solution is not more intensely coloured than a solution prepared at the same time by adding 1 ml of alkaline potassium Mercuri – iodide solution to a mixture of 4.0 ml of ammonium standard solution ( 1 ppm NH4 & 16.0 ml of ammonium free water (0.2 ppm)
Calcium & Magnesium in water: To 100 ml add 2 ml of ammonium buffer ph10.050 mg of mordant black II mixture & 0.5 ml of 0.01M disodium edentate, the pure blue colour is produced.
Heavy metals in water: Evaporate 150 ml to 15 ml on a water bath; 12 ml of the solution complies with the limit test for heavy metals, Method D (0.1 ppm). Use lead standard solution (1 ppm Pb ) to prepare the standard.
Chlorides in water: To 10 ml add 1 ml of 2 M Nitric Acid and 0.1M silver nitrate, the appearance of the solution does not change for at least 15 minutes.
Nitrates in water: To 5 ml in a test tube immersed in ice add 0.4 ml of diphenylamine solution & drop-wise with shaking, 5 ml of sulphuric acid. Transfer the tube to a water bath at 50 ° C & allow it to stand for 15 minutes. Any blue colour in the solution is not more intense than that in a solution prepared at the same time & in the same manner using a mixture of 4.5 ml of nitrate-free water & 0.5 ml of nitrate standard solution (2 ppm NO3) (0.2 ppm).
Sulphates in water: To 10 ml add 0.1 ml of 2 M hydrochloric acid & 0.1 ml of barium chloride solution. The appearance of the solution does not change for at least one 1hour.
Oxidisable substances in water: To 100 ml add 10 ml of 1 M sulphuric acid & 0.1 ml of 0.02 M potassium permanganate & boil for 5 minutes; the solution remains faintly pink.
Residue on evaporation of water: Evaporate 100 ml to dryness on a water bath & dry to constant weight at 105°.The residue weighs not more than
 1 mg (0.001%).
Ph:Â Â
5.0 to7.0
Conductivity: < 5µS/cm
6.0Â FREQUENCY:
6.1Â Â Daily basis.
7.0 ABBREBIATION
SOP:Â Â Â Â
Standard Operating Procedure
QA:Â Â Â Â Â
Quality Assurance
QC:Â Â Â Â Â
Quality Control
DM: Â Â DE-Mineralized
8.0 REFERENCE:
In House
SOP FOR OPERATION &Â REGENERATION OF DM PLANT